Volume Gel
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What volume do I need to load onto my gel so that I am using 0.5mg?
I want to load 0.5mg of a primer onto an electrophoresis gel
my stock solution is 100µM (mol.weight 6141 g/mol)
how much (µl) do I need to use to be loading 0.5mg onto the gel?
Please could you include calculations so that I can work it out myself in future, thanks!
100 uM = 100 umoles/liter. Since the MW = 6141, then 100 umoles will be 6141 x 100 = 614100 ugms. So this would be in 1000 mls or 614.1 ug/ml. This is equivalent to 0.6141 mg/ml. If you want 0.5 mg, you need just less than 1 ml, or 0.814 mls or 814 ul.
Beauties Factory - 20ml VOLUME DESIGN : Full Set Color Gel x 50 colors
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